Coding

Part:BBa_K1178000:Design

Designed by: Razan Alnahhas   Group: iGEM13_Greensboro-Austin   (2013-08-26)


tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1154
    Illegal BamHI site found at 1160
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1186
    Illegal NgoMIV site found at 1646
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 317
    Illegal SapI.rc site found at 1110


Design Notes

The synthetase gene is codon optimized for E. coli.


Source

Sequence from Alfonta et al. Site-specific incorporation of a redox-active amino acid into proteins. J Am Chem Soc. 2003 Dec 3;125(48):14662-3. PubMed PMID: 14640614.

References

  1. Alfonta et al. Site-specific incorporation of a redox-active amino acid into proteins. J Am Chem Soc. 2003 Dec 3;125(48):14662-3. PubMed PMID: 14640614.